Spectrophotomertry Essay Example | Topics and Well Written Essays - 1750 words

Spectrophotomertry - Essay Example 2. INTRODUCTION Spectrophotometry is the study of numeric calculation of the magnitude of reflection and transmittance of different materials on the basis of the incident wave length. A spectrophotometer is a device which is used to measure the values of transmittance and reflection. Spectrophotometry is used in different fields and sciences like chemistry, biochemistry, physics and materials etc. Spectrophotometry also covers the subject of spectrum and its intensity, and other related issues with changing thicknesses. This report is actually based on the application of spectrophotometry. Dektak 3 ST is used in this experiment. It is a surface profile interpreting mechanism, adequately well on to the area of measuring surface grain or texture and capable of measuring change in thickness even in microns. A diamond tip stylus is used to identify the sample characteristics. The device is enough responsive to record all surface variations. The parametric measurement through Dektak 3 ST includes area, slope, radius measurement, step height, unevenness and roughness on a sample profile. A set of data plotting and video imaging can also be retrieved by using Dektak 3 ST. All thin films on which the spectroscopic analysis is made are actually made up of tin oxide. Tin oxide is a colorless transparent, amphoteric and inorganic material. Tin oxide is widely used in ceramics and majorly in ceramics glazing. Tin oxide is also used in the production of glasses and enamels. 3. EXPERIMENT The experiment is comprised of two phases, the first one proceeds with enkd-8000 machine and the second one proceeds with Dektak 3ST. In the beginning of the phase 1 of the experiment three glasses of tin dioxide having three different thicknesses are considered. The entire glass top surfaces of all are determined with the help of voltmeter. Then every glass is taken individually and analyzed in enkd-8000 machine. During the analysis of every glass, the file is clicked with the scanning opt ion from 400 nm wavelength to 900 nm wavelength. After the selection of the wavelength the machine will take some time to analyze. Then after analysis is switched on in which performance summary information is selected. The selection will yield the CIE color space for the first glass. Now the whole process is being repeated for second and third glasses. All three analyses are made on P polarization When our analysis for P polarization is completed then any sample is selected for S polarization so a conclusion can be drawn by varying polarization. This is how the phase 1 of the experiment is completed. Considering the phase 2 of the experiment, all same three glasses of tin dioxide are used. Every glass is taken and then masked individually from the isolation tap then after zinc powder is being applied, then afterwards Hydrochloric acid is used for application on the applied zinc powder. Finally the hydrochloric acid is scrapped out and then samples are washed out. After this process if the glasses appear clean then apply acetone on it then again wash it with the help of water and then glass is being dried out. The same process is repeated for all three glasses. Dektak3ST machine is used to measure the thickness of all the samples of three glasses. The samples are positioned in the machine one by one. The run option is clicked by the mouse, and then scan option is clicked